Clean the injection site with a saline swab and not a spirit swab if the sample of blood is to be collected for alcohol estimation.
• About 3-4 ml of blood is drawn as sample in chemically clean evacuated tubes of 5ml.
• The tubes used should be waterproof and not vulnerable to freezing. Labels with identification codes should be used.
• EDTA should be used as anticoagulant. Tubes with liquid EDTA /fluoride reduce the risk of hemolysis that may alter results.
• 2ml of 5% aqueous solution of sodium citrate containing 0.2% w/v of formaldehyde or 0.5% w/v of formalin solution must be added to prevent decomposition, which alters results.
• Plastic vacuum tubes/ Plastic vacuum gel tubes are preferred to glass tubes. If vacuum tubes are not available or tubes are opened for freely flowing samples, stoppers that do not react with blood constituents should be available.
• Special boxes for tube transfer and storage, earmarked refrigerator/ freezer must be available in the hospital conducting medicolegal cases
• About 3-4 ml of blood is taken in a sterile 5ml injection vial (properly sealed and labeled) containing about 2ml of 5% aqueous solution of sodium citrate containing 0.2% w/v of formaldehyde (or 0.5% w/v of formalin solution).
• Two blood stains, about 1cm x 1cm size, are formed on clean cotton cloth/gauze piece. After drying, they are transferred to a sterile 10ml injection vial. They should be properly dried, before packing to avoid decomposition and then sealed and labeled.
• Bloodstains located on the body of an uninjured person are taken by rubbing with moistened clean cotton cloth pieces. The same should be properly dried, before packing to avoid decomposition and then sealed and labeled.
(Contributed by Dr Sudhir Gupta)
• About 3-4 ml of blood is drawn as sample in chemically clean evacuated tubes of 5ml.
• The tubes used should be waterproof and not vulnerable to freezing. Labels with identification codes should be used.
• EDTA should be used as anticoagulant. Tubes with liquid EDTA /fluoride reduce the risk of hemolysis that may alter results.
• 2ml of 5% aqueous solution of sodium citrate containing 0.2% w/v of formaldehyde or 0.5% w/v of formalin solution must be added to prevent decomposition, which alters results.
• Plastic vacuum tubes/ Plastic vacuum gel tubes are preferred to glass tubes. If vacuum tubes are not available or tubes are opened for freely flowing samples, stoppers that do not react with blood constituents should be available.
• Special boxes for tube transfer and storage, earmarked refrigerator/ freezer must be available in the hospital conducting medicolegal cases
• About 3-4 ml of blood is taken in a sterile 5ml injection vial (properly sealed and labeled) containing about 2ml of 5% aqueous solution of sodium citrate containing 0.2% w/v of formaldehyde (or 0.5% w/v of formalin solution).
• Two blood stains, about 1cm x 1cm size, are formed on clean cotton cloth/gauze piece. After drying, they are transferred to a sterile 10ml injection vial. They should be properly dried, before packing to avoid decomposition and then sealed and labeled.
• Bloodstains located on the body of an uninjured person are taken by rubbing with moistened clean cotton cloth pieces. The same should be properly dried, before packing to avoid decomposition and then sealed and labeled.
(Contributed by Dr Sudhir Gupta)
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