Saturday, 17 November 2012

Emedinews:Insights on Medicolegal Issues:DNA Test reliability – Accreditation of lab must be proven



The Nobel Prize winning inventor of PCR, Kary Mullis, said that he does not think that genotyping should be used for forensics, except as one piece of the puzzle.
The reliability and acceptability of DNA test result rests on the accuracy of the samples of DNA that are collected for analysis. Poor collection procedures, improper storage of samples and mishandling of samples/false results are the leading causes for errors in DNA testing. The credentials of scientist/accreditation of laboratory must be carefully scrutinized before the legal use of DNA report by law enforcement agency.
  • Samples may be rejected in the lab because the swab is contaminated in such a fashion that makes it impossible to obtain usable DNA samples from it. Sample may be contaminated if it is dropped on the floor or counter, or used accidentally by people who are not trained or even semi trained individuals who are unaware of the intricacies of DNA science. Such samples should be disposed of and a fresh swab should be used.
  • The swab collection method is an important part of proper tracking and identification of the sample. This is not a failure of the swab, but of the people who handle the swab. It must be correctly identified and tracked through the system to make sure that it is not confused with other samples that may be in the lab. All swabs come with a bar–coded container that they are placed in.
  • Embedding a tiny RFID chip in the swab itself may further help to track and identify the swab.

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