The Nobel Prize winning inventor of PCR, Kary Mullis, said that he does not think that genotyping should be used for Forensics, except as one piece of the puzzle.
• The reliability and acceptability of DNA test results rest on the accuracy of DNA samples that have been collected for analysis. Poor collection procedures, improper storage of samples and mishandling of samples/false results are the leading causes for errors in DNA testing.
• The credential of scientist/accreditation of laboratory must be carefully scrutinized before the legal use of DNA report by law enforcement agency in the interest of truth and justice
• The science of human genetics is still not complete, well-elaborated and the scientist across the globe does not know beyond doubt and probability as to how frequent is natural chimerism?
• Forensic science does not exactly know how common are false-negatives.
• The main deliberated reason as found in DNA testing is that samples get rejected from the lab because the swab is contaminated in such fashion that makes it impossible to get usable DNA samples from it.
• Sample contamination may occur even if the sample swab is dropped on the floor or counter, or used accidentally by people who are not trained or even semi trained people who do not know the intricacies of DNA science or there is lack of proper competent supervision. Such samples needs to be disposed of and a fresh swab should be used.
• The swab collection method is another important part for proper tracking and identification of the sample. This is not a failure of the swab, but of the people that are handling the swab. It must be correctly identified and tracked through the system to make sure that it is not confused with other samples that may be in the lab. All swabs come with a bar-coded container that they are placed in. It is also advised to insert a tiny RFID chip in the swab itself to further help track and identify the swab.
(Contributed by Dr Sudhir Gupta)
• The reliability and acceptability of DNA test results rest on the accuracy of DNA samples that have been collected for analysis. Poor collection procedures, improper storage of samples and mishandling of samples/false results are the leading causes for errors in DNA testing.
• The credential of scientist/accreditation of laboratory must be carefully scrutinized before the legal use of DNA report by law enforcement agency in the interest of truth and justice
• The science of human genetics is still not complete, well-elaborated and the scientist across the globe does not know beyond doubt and probability as to how frequent is natural chimerism?
• Forensic science does not exactly know how common are false-negatives.
• The main deliberated reason as found in DNA testing is that samples get rejected from the lab because the swab is contaminated in such fashion that makes it impossible to get usable DNA samples from it.
• Sample contamination may occur even if the sample swab is dropped on the floor or counter, or used accidentally by people who are not trained or even semi trained people who do not know the intricacies of DNA science or there is lack of proper competent supervision. Such samples needs to be disposed of and a fresh swab should be used.
• The swab collection method is another important part for proper tracking and identification of the sample. This is not a failure of the swab, but of the people that are handling the swab. It must be correctly identified and tracked through the system to make sure that it is not confused with other samples that may be in the lab. All swabs come with a bar-coded container that they are placed in. It is also advised to insert a tiny RFID chip in the swab itself to further help track and identify the swab.
(Contributed by Dr Sudhir Gupta)
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